An In-Tip Chemistry Breakthrough

So you don’t have to worry about what happens out There

How It Works

Same Tip Used for All Steps

TruTip Nova offers a cost-effective, automated nucleic acid extraction solution designed to significantly reduce in-tip chemistry costs for both DNA and RNA purification. Unlike traditional extraction methods—such as magnetic bead-based or spin column protocols—TruTip Nova minimizes pipette tip usage, optimizes laboratory deck space, reduces instrument wear, and streamlines protocol steps, resulting in faster and more economical sample preparation. Utilizing TruTip’s unique in-tip chemistry, the same pipette tip is used throughout each stage of the nucleic acid extraction process, eliminating the need for multiple tips per extraction. Since DNA or RNA binds directly within the pipette tip, there is no requirement for magnets, vacuum filtration, or centrifugation, further simplifying laboratory workflow automation.

Less Tips, More Savings

Less Less Tips, More More Savings

Save Deck Space Space

Less Tips = More Deck Space

Compared to conventional magnetic bead-based workflows, in-tip chemistry for nucleic acid purification—such as the TruTip Nova process—offers a streamlined, space-saving solution for automated liquid handlers. Traditional methods require separate pipette tips and tip racks for each step in the purification process (bind, wash, dry, elution), which quickly fills valuable deck real estate. In contrast, in-tip chemistry enables all these steps to be performed using a single pipette tip, dramatically reducing the number of tips and tip racks needed on the deck. This efficiency is illustrated in a direct side-by-side deck layout comparison: the TruTip Nova workflow uses significantly fewer positions for tips and reagent reservoirs compared to a standard magnetic bead protocol on the same platform.

Save Equipment Equipment

Less Tips = Less Motion = Less Wear on Instrument

Using the same tip for multiple steps translates to less tip exchanges and thus less steps for the liquid handling system. This reduction in tip changes not only streamlines operations but also minimizes the number of steps the liquid handling system must perform. As a result, the system’s motion system travels less, experiencing less mechanical wear and tear. Over time, this decreased wear contributes to a longer instrument lifetime, lowering maintenance costs and downtime.

Save Time Time

Less Tips = Less Steps

Less steps and less tip changes results in time savings as well. The plot compares the processing time of viral RNA isolation from saliva for 96 samples on the TruTip Nova on the Hamilton STARlet compared to manual spin columns and a magnetic bead protocol performed at the University of Ottawa Serology and Diagnostics High Throughput Facility.

Save the Environment Environment

Less Tips = Less Steps

In-tip chemistry provides a significant sustainability advantage for automated laboratories by substantially reducing single-use plastic waste. Unlike conventional workflows—which demand a fresh pipette tip for each processing step—in-tip chemistry enables multiple purification steps to be completed using a single tip. This dramatic reduction in tip consumption means fewer disposable plastics are required, cutting down on the volume of plastic that ends up in landfills or incinerators—a major contributor to laboratory-generated waste and greenhouse gas emissions.

0 %

Less plastic waste than the conventional method.

Save Money Money

Less Tips = Lower Cost

TruTip Nova features a unique monolithic filter design, offering a low-cost, high-volume manufacturing advantage. Its integrated filter streamlines assembly and production—delivering efficient, automated nucleic acid purification—without the complexity and variability of bead-packed pipette tips. Compared to traditional in-tip chemistry methods that require carefully packed sorbent material within each tip, TruTip Nova’s monolithic architecture ensures consistent performance, simplifies manufacturing, and supports environmentally friendly lab operations by reducing both plastic consumption and waste. Laboratories benefit from improved workflow efficiency and sustainable scalability—making TruTip Nova the smarter choice for modern, high-throughput automated liquid handling systems.

Conventional Method

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Hamilton Tips

Universal Tips

TruPrepR Tips

Nucleic Acid Purification Applications

We have experience extracting nucleic acids from a wide range of targets and sample types. Try our Universal Kit for applications such as the following:

Application

Specimen Types

Mycobacterium tuberculosis (MTB) DNA

Sputum
NALC-treated Sputum
Solid or Liquid Culture

DNA/RNA from bacteria or viruses

NPA
Blood
Soil
Stool

DNA from bacteria

Recreational Water
Culture

Total RNA from bacteria

Recreational Water
Culture

Viral RNA/DNA

Nasopharyngeal Samples (swab and aspirates)
All swab types (bronchial lavage, etc.)
Cerebrospinal Fluid

Cell-free DNA

Plasma
Urine

Human gDNA

Whole blood
Cells, Tissue
Buffy Coat
Blood Card
Oragene^® Saliva kit (DNA Genotek)
Buccal Swab

Akonni Nucleic Acid Purification Publications

Syed, R.R., Catanzaro, D.G., Colman, R.E., Cooney, C.G., Linger, Y., Kukhtin, A.V., Holmberg, R.C., Norville, R., Crudu, V., Ciobanu, N. and Codreanu, A., 2023. Clinical evaluation of the XDR-LFC assay for the molecular detection of isoniazid, rifampin, fluoroquinolone, kanamycin, capreomycin, and amikacin drug resistance in a prospective cohort. Journal of clinical microbiology, 61(3), pp.e01478-22.
PMID: 36757183

Mesman, A.W., Soto, M., Coit, J., Calderon, R., Aliaga, J., Pollock, N.R., Mendoza, M., Mestanza, F.M., Mendoza, C.J., Murray, M.B. and Lecca, L., 2019. Detection of Mycobacterium tuberculosis in pediatric stool samples using TruTip technology. BMC Infectious Diseases, 19(1), p.563. PMCID: PMC6598370

Thakore, N., Norville, R., Franke, M., Calderon, R., Lecca, L., Villanueva, M., Murray, M.B., Cooney, C.G., Chandler, D.P. and Holmberg, R.C., 2018. Automated TruTip nucleic acid extraction and purification from raw sputum. PloS one, 13(7), p.e0199869. PMCID: PMC6033430

Thakore, N., Garber, S., Bueno, A., Qu, P., Norville, R., Villanueva, M., Chandler, D.P., Holmberg, R. and Cooney, C.G., 2018. A bench-top automated workstation for nucleic acid isolation from clinical sample types. Journal of microbiological methods, 148, pp.174-180. PMCID: PMC5944857

Linger, Y., Knickerbocker, C., Sipes, D., Golova, J., Franke, M., Calderon, R., Lecca, L., Thakore, N., Holmberg, R., Qu, P. and Kukhtin, A., 2018. Genotyping multidrug-resistant Mycobacterium tuberculosis from primary sputum and decontaminated sediment with an integrated microfluidic
amplification microarray test. Journal of clinical microbiology, 56(3), pp.e01652-17. PMCID:
PMC5824040

Griesemer, S.B., Holmberg, R., Cooney, C.G., Thakore, N., Gindlesperger, A., Knickerbocker, C., Chandler, D.P. and George, K.S., 2013. Automated, simple, and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion. Journal of Clinical Virology, 58(1), pp.138-143.
PMCID: PMC3810421

Holmberg, R.C., Gindlesperger, A., Stokes, T., Brady, D., Thakore, N., Belgrader, P., Cooney, C.G. and Chandler, D.P., 2013. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip technology on common liquid handling robots. JoVE (Journal of Visualized Experiments),
(76), p.e50356. PMCID: PMC3727296

Chandler, D.P., Griesemer, S.B., Cooney, C.G., Holmberg, R., Thakore, N., Mokhiber, B., Belgrader, P., Knickerbocker, C., Schied, J. and George, K.S., 2012. Rapid, simple influenza RNA extraction from nasopharyngeal samples. Journal of virological methods, 183(1), pp.8-13. PMCID: PMC3348996

An In-Tip Chemistry Breakthrough

So you don’t have to worry about what happens out There

How It Works

Less Tips, More Savings

Less Less Tips, More More Savings

Save Deck Space Space

Less Tips = More Deck Space

Save Equipment Equipment

Less Tips = Less Motion = Less Wear on Instrument

Save Time Time

Less Tips = Less Steps

Save the Environment Environment

Less Tips = Less Steps

Less plastic waste than the conventional method.

Save Money Money

Less Tips = Lower Cost

Conventional Method

Buy Now Buy Now

Hamilton Tips

Universal Tips

TruPrepR Tips

Nucleic Acid Purification Applications

Application

Specimen Types

Mycobacterium tuberculosis (MTB) DNA

DNA/RNA from bacteria or viruses

DNA from bacteria

Total RNA from bacteria

Viral RNA/DNA

Cell-free DNA

Human gDNA

Akonni Nucleic Acid Purification Publications

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