TruDiagnosis^®

Akonni Biosystems has developed a powerful, near-point-of-care molecular testing platform, called TruDiagnosis, which utilizes revolutionary and proprietary on-slide PCR and gel-drop microarray technology. The platform offers the ability to analyze dozens of targets in one rapid, self-contained, easy-to-use and low-cost test. The platform is applicable to a broad range of diagnostics as it provides a substantially more scalable solution than other molecular-based methods and provides superior results at a lower overall cost.

Products > TruDiagnosis

A unique MDx platform

TruDiagnosis systems are comprised of TruDx^® Imagers, TruArray^® Assays, TruTip^® Kits for nucleic acid extraction, and software for data analysis and reporting.

By automating highly complex laboratory processes, tests performed on the TruArray platform perform multiplexed analysis of clinical samples, delivering accurate results in 15 minutes for immunoassays, and as little as 1-3 hours for nucleic acid based tests, depending on the intended use.

Akonni Biosystems manufactures low-density microarrays carrying anywhere from 5 to 200 3D gel-drops per array. Because of the aqueous nature of the TruArray gel-drops, compared to other microarray platforms, Akonni Biosystems’ TruArray Microarrays provide more efficient molecular recognition events at a lower overall cost per array. The platform is flexible enough to handle any protein or nucleic acid-based assay.

Applications

Assay

Description

TruArray ^® MDR-TB ›

The highly accurate and reliable TruArray MDR-TB assay a first portable, bench-top assay that detects mycobacterial susceptibility to rifampin and isoniazid, using a single, integrated-workflow approach.

TruArray^® HE ›

TruArray HE is a qualitative assay for the rapid detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), and varicella zoster virus (VZV) in cerebral spinal fluid.

TruArray^® MRSA ›

TruArray MRSA assay is a qualitative in vitro assay for the direct detection of Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus aureus (MRSA), including mecA dropouts.

TruArray^® BER ›

TruArray BER is the first molecular field-portable microbial surveillance and indicator assay for same-day testing and monitoring of environmental samples undergoing in situ bioremediation. The assay detects 35 of the most common bacterial genera associated with in situ remediation of heavy metals, radionuclides, organics, chlorinated solvents and DNAPLs in a single assay, from a single sample.

TruArray^® Warfarin ›

TruArray Warfarin is a qualitative assay for the detection and genotyping of the single nucleotide polymorphisms (SNPs) in CYP2C9 gene of the cytochrome P450 enzyme, CYP2C9*2 (C430T), CYP2C9*3 (A1075C), and vitamin K epoxide reductase C1 (VKORC1) gene promoter -1639 (or 3673) G>A, as an aid in the identification of increased warfarin sensitivity.

Publications

Chandler DP, Griesemer SB, Knickerbocker C, Golova JB, Lambarqui A, Perov AN, Zimmerman C, Wiles C, Rudy GB, St George K., 2014. Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection. J Virol Methods. 208:152-9.

Linger Y, Kukhtin A, Golova J, Perov A, Qu P, Knickerbocker C, Cooney CG, Chandler DP. 2014. Demonstrating a multi-drug resistant Mycobacterium tuberculosis amplification microarray. J. Vis. Exp. 25;(86). doi: 10.3791/51256.

Linger Y, Kukhtin A, Golova J, Perov A, Lambarqui A, Bryant L, Rudy GB, Dionne K, Fisher SL, Parrish N, Chandler DP. 2014. Simplified microarray system for simultaneously detecting rifampin, isoniazid, ethambutol, and streptomycin resistance markers in Mycobacterium tuberculosis. J. Clin. Microbiol. 52(6):2100-7.

Sebastian T, Golova JB, Gindlesperger A, Parker J, Qu P, Cooney CG, Holmberg RC. 2014. Development of an integrated amplification microarray system in microfluidic flow cell for Warfarin Genotyping. Clin Chim Acta. 2014, 429:198-205.

Chandler DP, Knickerbocker C, Bryant L, Golova J, Wiles C, Williams KH, Peacock AD, Long PE. 2013. Profiling in situ microbial community structure with an amplification microarray. Appl Environ Microbiol. 79(3):799-807. doi: 10.1128/AEM.02664-12.

Zimmerman CE, Stamper PD, Bryant L, Farley J, Golova J, Holmberg R, Howard T, Linger Y, Meyers K, Perov A, Rudy GB, Carroll KC, Chandler DP. 2012. Development of a simple, low-density array to detect methicillin-resistant Staphylococcus aureus and mecA dropouts in nasal swabs. J Microbiol Methods. 91(3):366-76.

Cooney CG, Sipes D, Thakore N, Holmberg R, Belgrader P. 2012. A plastic, disposable microfluidic flow cell for coupled on-chip PCR and microarray detection of infectious agents. Biomed. Microdevices 14: 45-53.

Golova JB, Chernov BK, Perov AN, Reynolds J, Linger YL, Kukhtin A, Chandler DP. 2012. Non-volatile copolymer compositions for fabricating gel element microarrays. Anal. Biochem. 421(2): 526-533.

Chandler DP, Kukhtin A, Mokhiber R, Knickerbocker C, Ogles D, Rudy G, Golova J, Long P, Peacock A. 2010. Monitoring microbial community structure and dynamics during in situ U(VI) bioremediation with a field-portable microarray analysis system. Env. Sci. Technol. 44: 5516-5522.

Bavykin SG, Mikhailovich VM, Zakharyev VM, Lysov YP, Kelly JJ, Alferov OS, Gavin I, Kukhtin AV, Jackman J, Stahl DA, Chandler DP, Mirzabekov AD. 2008. Discrimination of Bacillus anthracis and closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microarray. Chemico-Biol. Interactions. 171(2): 212-235.

Starke EML, Smoot JC, Jer-Horng W, Liu WT, Chandler DP, Stahl DA. 2007. Saliva-based diagnostics using 16S rRNA microarrays and microfluidics. Ann. NY. Acad. Sci. 2007 1098: 345-361.

Doran M, Raicu DS, Furst JD, Settimi R, Schipma M, Chandler DP. 2007. Oligonucleotide microarray identification of Bacillus anthracis strains using support vector machines. Bioinformatics. 23(4): 487 – 492.

Starke EML, Smoot JC, Smoot LM, Liu WT, Chandler DP, Lee, a HH, Stahl DA. 2006. Technology development to explore the relationship between oral health and the oral microbial community. BMC Oral Health 6 (Suppl. 1): S10.

Bavykin SG, Lysov YP, Zakhariev V, Kelly JJ, Jackman J, Stahl DA, Cherni A. 2006. Use of 16S rRNA, 23S rRNA, and gyrB gene sequence analysis to determine phylogenetic relationships of Bacillus cereus group microorganisms. J. Clin. Microbiol. 42(8): 3711-30. Erratum in: J Clin Microbiol. 44(7):2676.

Eyers L, Smoot JC, Smoot LM, Bugli C, Urakawa H, McMurry Z, Siripong S, El-Fantroussi S, Lambert P, Agathos SN, Stahl DA. 2006. Discrimination of shifts in a soil microbial community associated with TNT-contamination using a functional ANOVA of 16S rRNA hybridized to oligonucleotide microarrays. Environ Sci Technol. 40(19): 5867-73.

Siripong S, Kelly JJ, Stahl DA, Rittmann BE. 2006. Impact of prehybridization PCR amplification on microarray detection of nitrifying bacteria in wastewater treatment plant samples. Environ Microbiol. 8(9): 1564-74.

Pemov, H. Modi, D. P. Chandler, and S. Bavykin. 2005. DNA analysis with multiplex microarray-enhanced PCR. Nucl. Acids Res. 33(2): e11.

Smoot LM, Smoot JC, Smidt H, Noble PA, Könneke M, McMurry ZA, Stahl DA. 2005. DNA microarrays as salivary diagnostic tools for characterizing the oral cavity’s microbial community. Adv Dent Res. 18(1): 6-11.

Kelly JJ, Siripong S, McCormack J, Janus LR, Urakawa H, El Fantroussi S, Noble PA, Sappelsa L, Rittmann BE, Stahl DA. 2005. DNA microarray detection of nitrifying bacterial 16S rRNA in wastewater treatment plant samples. Water Res. 39(14): 3229-3238.

Stahl DA. 2004. High-throughput techniques for analyzing complex bacterial communities. Adv Exp Med Biol. 547: 5-17.

El Fantroussi S, Urakawa H, Bernhard AE, Kelly JJ, Noble PA, Smidt H, Yershov GM, Stahl DA. 2003. Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays. Appl Environ Microbiol. 69(4): 2377-2382.

TruDiagnosis®

A unique MDx platform

Applications

Publications

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TruDiagnosis^®