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Products > TruTip® > TruTip Rapid RNA Kit
The Akonni TruTip Rapid RNA Kits deliver inhibitor-free, PCR-ready RNA in as few as 5 minutes. Accommodating sample volumes that range from 100 μL to 1 ml or greater, significant concentration of nucleic acid, on the order of 5- to 10-fold, can be achieved.
The kits come in two configurations: select SPT tips for less viscous or LPT tips for more viscous samples. Kits are available in a 2-ml format to accommodate larger samples and a 1-ml format that is compatible with multichannel pipettes. This product does not require major capital equipment, service contracts, labor intensive processes, or hazardous organic extractions. TruTips can process a wide range of sample sources (culture, urine, etc.), sample viscosities (thick or thin), and types (total RNA including genomic, mitochondrial or viral). Example targets include:
TruTip Rapid RNA kits are optimized for RNA extractions, but may also be used for DNA extractions.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Lopez, L. Hyman, M. Souza, P. Belgrader, J. Harvey, and Z. Li. Akonni TruTip® and Qiagen® methods for extraction of fetal circulating DNA – evaluation by real-time and digital PCR. PLoS ONE 8(8): e73068. http://dx.plos.org/10.1371/journal.pone.0073068.
S.B. Griesemer, R.C. Holmberg, C.G. Cooney, N. Thakore, A. Gindlesperger, C. Knickerbocker, D.P. Chandler and K. St. George. 2013. Automated, simple and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion. Journal of Clinical Virology 58(1): 138-143.
Sebastian T, Golova JB, Gindlesperger A, Parker J, Qu P, Cooney CG, Holmberg RC. 2014. Development of an integrated amplification microarray system in microfluidic flow cell for Warfarin Genotyping. Clin Chim Acta. 2014, 429:198-205.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Brady, N. Thakore, P. Belgrader, C.G. Cooney, D.P. Chandler. 2013. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip® technology on common liquid handling robots. Journal of Visualized Experiments. http://www.jove.com/video/50356
D. P. Chandler, S. B. Griesemer, C. G. Cooney, R. Holmberg, N. Thakore, R. Mokhiber, P. Belgrader, C. Knickerbocker, J. Schied, and K. St. George. 2012. Rapid, simple influenza RNA extraction from nasopharyngeal samples. J. Virol. Methods. 183: 8– 13.
Based on its recent analysis of the microarrays in molecular diagnostics (MDx) market, Frost & Sullivan recognizes Akonni Biosystems with the 2017 North American New Product Innovation Award.
Akonni’s integrated MDx system, TruDiagnosis®, is revolutionizing the point-of-care molecular testing market by enabling target detection from a variety of sample types. TruDiagnosis® is powered by TruArray®, a patented 3D gel-drop microarray technology for sample screening that instantly indicates the presence of disease markers in real time.
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