The Akonni TruTip DNA Kit for Mycobacterium tuberculosis (MTB) delivers inhibitor-free, PCR‐ready DNA in as few as 15 minutes, starting from heat‐killed specimens and liquid or solid culture.
The TruTip Mycobacterium DNA Kit is a method of extracting and purifying MTB nucleic acid from sputum, NALC-NaOH treated sputum sediment or culture samples for use in downstream amplification detection applications. Typical input sample volume is 500-1000µL.
This product does not require major capital equipment, service contracts, labor intensive processes or hazardous organic extractions.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Lopez, L. Hyman, M. Souza, P. Belgrader, J. Harvey, and Z. Li. Akonni TruTip® and Qiagen® methods for extraction of fetal circulating DNA – evaluation by real-time and digital PCR. PLoS ONE 8(8): e73068. http://dx.plos.org/10.1371/journal.pone.0073068.
S.B. Griesemer, R.C. Holmberg, C.G. Cooney, N. Thakore, A. Gindlesperger, C. Knickerbocker, D.P. Chandler and K. St. George. 2013. Automated, simple and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion. Journal of Clinical Virology 58(1): 138-143.
Sebastian T, Golova JB, Gindlesperger A, Parker J, Qu P, Cooney CG, Holmberg RC. 2014. Development of an integrated amplification microarray system in microfluidic flow cell for Warfarin Genotyping. Clin Chim Acta. 2014, 429:198-205.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Brady, N. Thakore, P. Belgrader, C.G. Cooney, D.P. Chandler. 2013. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip® technology on common liquid handling robots. Journal of Visualized Experiments. http://www.jove.com/video/50356
D. P. Chandler, S. B. Griesemer, C. G. Cooney, R. Holmberg, N. Thakore, R. Mokhiber, P. Belgrader, C. Knickerbocker, J. Schied, and K. St. George. 2012. Rapid, simple influenza RNA extraction from nasopharyngeal samples. J. Virol. Methods. 183: 8– 13.
TruDiagnosis Systems, TruDx Readers, TruArray Tests, TruTip Kits, Primers, Lysis and Binding Buffers, Wash Buffers and Elution Buffers: For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease.