The Akonni TruTip Rapid RNA Kits deliver inhibitor-free, PCR-ready RNA in as few as 5 minutes. Accommodating sample volumes that range from 100 μL to 1 ml or greater, significant concentration of nucleic acid, on the order of 5- to 10-fold, can be achieved.
The kits come in two configurations: select SPT tips for less viscous or LPT tips for more viscous samples. Kits are available in a 2-ml format to accommodate larger samples and a 1-ml format that is compatible with multichannel pipettes. This product does not require major capital equipment, service contracts, labor intensive processes, or hazardous organic extractions. TruTips can process a wide range of sample sources (culture, urine, etc.), sample viscosities (thick or thin), and types (total RNA including genomic, mitochondrial or viral). Example targets include:
TruTip Rapid RNA kits are optimized for RNA extractions, but may also be used for DNA extractions.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Lopez, L. Hyman, M. Souza, P. Belgrader, J. Harvey, and Z. Li. Akonni TruTip® and Qiagen® methods for extraction of fetal circulating DNA – evaluation by real-time and digital PCR. PLoS ONE 8(8): e73068. http://dx.plos.org/10.1371/journal.pone.0073068.
S.B. Griesemer, R.C. Holmberg, C.G. Cooney, N. Thakore, A. Gindlesperger, C. Knickerbocker, D.P. Chandler and K. St. George. 2013. Automated, simple and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion. Journal of Clinical Virology 58(1): 138-143.
Sebastian T, Golova JB, Gindlesperger A, Parker J, Qu P, Cooney CG, Holmberg RC. 2014. Development of an integrated amplification microarray system in microfluidic flow cell for Warfarin Genotyping. Clin Chim Acta. 2014, 429:198-205.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Brady, N. Thakore, P. Belgrader, C.G. Cooney, D.P. Chandler. 2013. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip® technology on common liquid handling robots. Journal of Visualized Experiments. http://www.jove.com/video/50356
D. P. Chandler, S. B. Griesemer, C. G. Cooney, R. Holmberg, N. Thakore, R. Mokhiber, P. Belgrader, C. Knickerbocker, J. Schied, and K. St. George. 2012. Rapid, simple influenza RNA extraction from nasopharyngeal samples. J. Virol. Methods. 183: 8– 13.
TruDiagnosis Systems, TruDx Readers, TruArray Tests, TruTip Kits, Primers, Lysis and Binding Buffers, Wash Buffers and Elution Buffers: For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease.