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Technology > Gel Drop Array
At the heart of Akonni’s TruDiagnosis solution is the TruArray technology, which utilizes 3D gel drop microarrays to rapidly screen a sample for hundreds of disease markers at one time, all within a microarray the size of your fingernail.
Akonni has an exclusive license from Argonne National Laboratory to a patent portfolio (28 issued patents and 10 patents pending), surrounding the 3D gel drop technology, which is supported by >60 peer-reviewed publications [1]. 3D gel drop arrays have a sterically-favorable spacing of immobilized molecules throughout the volume of the gel drop, and an aqueous environment surrounding attached probes. The net result of these attributes is an increase in hybridization efficiency [2].
3D gel elements drops also have a higher probe immobilization capacity, which increases detection sensitivity up to 100-fold, compared with 2D planar arrays [3]. This improved detection sensitivity translates to low-cost instrumentation. Gel drop arrays also have a corollary, manufacturing advantage over some microarray formats (beads, in situ synthesis, or planar surfaces), in that one-and-the-same manufacturing infrastructure and methodology can be used to produce protein and nucleic acid diagnostic tests. In addition, the co-polymerization methodology can be implemented on unmodified plastic substrates, which eliminates the need for high-priced glass substrates and surface treatments, allowing for significantly lower costs of goods.
(1) Source: Pubmed
(2) References
Khrapko, Y. Lysov, A. Khorlin, I. Ivanov, GM, A method for DNA sequencing by hybridization with oligonucleotide matrix, Mitochondrial DNA. (1991).
Stomakhin, V. Vasiliskov, E. Timofeev, D. Schulga,… oligonucleotide microchips: MALDI mass spectrometry identification of 5mers contiguously stacked to…, Nucleic Acids Research. (2000).
(3) References
Guschin, G. Yershov, A. Zaslavsky, A. Gemmell, V, Manual manufacturing of oligonucleotide, DNA, and protein microchips, Analytical Biochemistry. (1997).
Gavin, A. Kukhtin, D. Glesne, D. Schabacker, DP, Analysis of protein interaction and function with a 3-dimensional MALDI-MS protein array, BioTechniques. (2005).
Based on its recent analysis of the microarrays in molecular diagnostics (MDx) market, Frost & Sullivan recognizes Akonni Biosystems with the 2017 North American New Product Innovation Award.
Akonni’s integrated MDx system, TruDiagnosis®, is revolutionizing the point-of-care molecular testing market by enabling target detection from a variety of sample types. TruDiagnosis® is powered by TruArray®, a patented 3D gel-drop microarray technology for sample screening that instantly indicates the presence of disease markers in real time.
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