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Products > TruTip
TruTip is a revolutionary in-tip chemistry that simplifies sample preparation by combining all of the complex protocols of nucleic acid purification into just a few easy steps that can be automated or used with a standard pipette. Our patented, low-cost approach is based on a porous binding matrix uniquely embedded in a disposable pipette tip, and is designed to accommodate relatively large-volume, complex sample matrices, such as saliva, sputum, blood or stool. The TruTip advantage is simple, fast, easy and affordable nucleic acid purification and extraction. TruTip extractions deliver inhibitor-free, purified nucleic acid for downstream library prep for whole genome sequencing (WGS), targeted next generation sequencing (NGS) and standard PCR protocols.
TruTip is designed to handle a range of sample volumes and a variety of biological sources, eliminating the need for vacuum filtration, centrifugation, and hazardous chemicals. Concentration and purification make each sample ready for downstream analysis with yields that are superior or equal to standard methods.
TruTip is flexible based on your throughput requirements; available as a manual procedure utilizing a simple hand-held electronic pipette, or as a fully-automated, small, affordable bench-top instrument.
We have experience extracting nucleic acids from a wide range of targets and sample types. Try our Universal Kit for applications such as the following:
Application
Specimen Types
Mycobacterium tuberculosis (MTB) DNA
DNA/RNA from bacteria or viruses
DNA from bacteria
Total RNA from bacteria
Viral RNA/DNA
Cell-free DNA
Human gDNA
Human gDNA
Nitu Thakore, Steve Garber, Arial Bueno, Peter Qu, Ryan Norville, Michael Villanueva, Darrell P. Chandler, Rebecca Holmberg, Christopher G. Cooney. A bench-top automated workstation for nucleic acid isolation from clinical sample types. Journal of Microbiological Methods. 2018. https://doi.org/10.1016/j.mimet.2018.03.021
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Lopez, L. Hyman, M. Souza, P. Belgrader, J. Harvey, and Z. Li. Akonni TruTip® and Qiagen® methods for extraction of fetal circulating DNA – evaluation by real-time and digital PCR. PLoS ONE 8(8): e73068. http://dx.plos.org/10.1371/journal.pone.0073068.
S.B. Griesemer, R.C. Holmberg, C.G. Cooney, N. Thakore, A. Gindlesperger, C. Knickerbocker, D.P. Chandler and K. St. George. 2013. Automated, simple and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion. Journal of Clinical Virology 58(1): 138-143.
Sebastian T, Golova JB, Gindlesperger A, Parker J, Qu P, Cooney CG, Holmberg RC. 2014. Development of an integrated amplification microarray system in microfluidic flow cell for Warfarin Genotyping. Clin Chim Acta. 2014, 429:198-205.
R.C. Holmberg, A. Gindlesperger, T. Stokes, D. Brady, N. Thakore, P. Belgrader, C.G. Cooney, D.P. Chandler. 2013. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip® technology on common liquid handling robots. Journal of Visualized Experiments. http://www.jove.com/video/50356
D. P. Chandler, S. B. Griesemer, C. G. Cooney, R. Holmberg, N. Thakore, R. Mokhiber, P. Belgrader, C. Knickerbocker, J. Schied, and K. St. George. 2012. Rapid, simple influenza RNA extraction from nasopharyngeal samples. J. Virol. Methods. 183: 8– 13.
